MicroRNA-1 Regulates the Differentiation of Adipose-Derived Stem Cells into Cardiomyocyte-Like Cells

Figure 5

Expression levels of Notch1, Hes1, cTnI, and GATA4 proteins in ASCs after coculture with CMs. Three groups of 3rd-generation lentivirus (Lv)-infected ASCs were examined as follows: (1) Lv-miR-1-CMs (miRNA-1-expressing cells cocultured with CMs), (2) Lv-miR-1 (miRNA-1-expressing ASCs), and (3) Lv-NC-CMs (control infected ASCs cocultured with CMs). (a) Western blot analysis of the indicated proteins. GAPDH was used as normalization control. (b–e) Quantification of cTnI, GATA4, Notch1, and Hes1 protein levels, as indicated. (f–i) mRNA expression levels of cTnI, GATA4, Notch1, and Hes1, as indicated. and .

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