Human Adipose Mesenchymal Stem Cells Show More Efficient Angiogenesis Promotion on Endothelial Colony-Forming Cells than Umbilical Cord and Endometrium

Figure 2

Characterization of multipotent mesenchymal stem/stromal cells (MSCs) derived from adipose, umbilical cord, and endometrium tissues. (a) Morphology of cultured adipose-derived stromal cells (ADSCs), umbilical cord mesenchymal stromal cells (UCMSCs), and endometrium mesenchymal stromal cells (EMSCs) in passage 4. Top panels and bottom panels are images at low (×40) and high (×100) magnification, respectively. All MSCs exhibited homogeneous spindle-shaped morphology. (b) Cytometric analysis of cultured MSCs in P0 and P4. Cells were labeled with antibodies against CD29, CD90, CD34, CD45, CD31, CD14, CD19, HLA-DR, CD73, and CD105 (solid red histograms). Black-lined histograms represent isotype-matched controls. (c) Multilineage differentiation potential of ADSCs, UCMSCs, and EMSCs in passage 4. Adipogenesis was assessed by staining cultures with Oil Red O to measure the accumulation of lipid vacuoles. Osteogenesis was assessed in alkaline phosphatase staining. Chondrogenesis was assessed by Alcian blue staining to detect the synthesis of proteoglycans. All MSCs showed multipotential differentiation capability.

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