Human Adipose Mesenchymal Stem Cells Show More Efficient Angiogenesis Promotion on Endothelial Colony-Forming Cells than Umbilical Cord and Endometrium

Figure 5

The therapeutic efficacy of different cells in murine hindlimb ischemia model. (a) Perfusion heat maps of different cell groups on day 0, 7, 14, 21, and 28, respectively. Different MSCs with ECFCs were transplanted by tail intravenous injection 24 h after right femoral and saphenous artery ligation. Laser Doppler Perfusion Imaging (LDPI) visualized the dynamic changes in hind limb perfusion of different cytotherapy groups on indicated time points. Normal saline (NS) was used as control. (b) Blood perfusion rate of different cell groups. Blood perfusion was quantified using perfusion rate, i.e., the rate of average LDPI index of ischemic limb (left) to nonischemic hind limb (right). Bars represent the mean perfusion rate ± SD. , . . NS was used as control. (c) Representative images of five progressive exterior morphological recovery levels of ischemic mice on day 28. From left to right are limb salvage, bloated foot, amyotrophy, mild loss of limb, and severe loss of limb, respectively (yellow arrow). (d) Percentage bar chart of exterior recovery statistics in different cytotherapy groups. Kruskal-Wallis H ANOVA was used to analyze the composition. NS was used as control. .

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