Transplantation of Hypoxic-Preconditioned Bone Mesenchymal Stem Cells Retards Intervertebral Disc Degeneration via Enhancing Implanted Cell Survival and Migration in Rats

Figure 1

(a) Morphology of BMSCs was observed under microscope (200×). (b) BMSC-GFP displayed green fluorescence and blue nuclei under fluorescence microscopy (100×). (c) Osteogenic differentiation of BMSCs: dark brown calcium nodules stained by alizarin red were observed in cells (400×). (d) Adipogenic differentiation of BMSCs: brown lipid droplets stained by oil red O were observed in cells (400×). (e–h) The P3 BMSC-positive rates of CD29, CD90, CD31, and CD45 were detected by FCM.

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