Differentiation Potential of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells and Paracrine Signaling Interaction Contribute to Improve the In Vitro Maturation of Mouse Cumulus Oocyte Complexes

Table 1

Developmental competency of COCs among the different culture systems.

Culture media	Total number of COCs	Polar bodies (number)	Polar bodies (%)	Number of cleaved oocytes (%)	Number of blastocyst stage (%)
DMEM-F12	199	81	40.7
G1-PLUS	204	85	41.6
G2-PLUS	199	115	57.8	61 (53.04)	7 (11.48)
TYH	198	71	35.9
Conditioned-DMEM-F12	200	56	28
Conditioned-G1-PLUS	206	79	38.4
Conditioned-G2-PLUS	204	69	33.8
Conditioned-TYH	202	81	40.1
Coculture-DMEM-F12	205	115	56.1	58 (50.43)	8 (13.79)
Coculture-G1-PLUS	203	51	25.1
Coculture-G2-PLUS	206	66	32
Coculture-TYH	196	74	37.7

Oocytes with polar bodies were considered mature MII oocytes. The fertilization rate and rate of development to the blastocyst stage were compared between G2-PLUS and coculture-DMEM-F12. Both showed no significant differences in their ability to improve oocyte maturation and were statistically different from the rest of the systems. The data were analyzed by the chi-squared test. A value of was considered statistically significant.