TGF-β Inhibitor SB431542 Promotes the Differentiation of Induced Pluripotent Stem Cells and Embryonic Stem Cells into Mesenchymal-Like Cells

Figure 3

Flow cytometry dot plots showing the coexpression of CD44 and CD29 in GENEA 02-MSC6 (a), H9-MSC4 (b), and H9 OCT4 mCHERRY-MSC4 (c) cells. The gates denoting positive expression of CD44 and CD29 were selected by exclusion of greater than 99% of events appearing in the isotype control pattern. CD44 and CD29 were found to be strongly coexpressed in both iPSC- and ESC-derived MSC lines examined. Each plot represents 10.000 FACS events.

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