TGF-β Inhibitor SB431542 Promotes the Differentiation of Induced Pluripotent Stem Cells and Embryonic Stem Cells into Mesenchymal-Like Cells

Figure 6

Morphological characteristics of GENEA 02-MSC5 (a), NF1-MSC3 (b), and BM-hMSCs (c) after 31 days of directed osteogenic differentiation. Both NF1-MSC3 and BM-hMSCs displayed a strong osteogenic differentiation ability as indicated by the presence of a mineralized matrix in the culture. GENEA 02-MSC5 cells, however, showed a limited osteogenic differentiation ability when compared with NF1-MSC3 cells. (d) Average calcium concentration in BM-hMSCs and ESC-derived MSCs after 31 days of culture in osteogenic differentiation media or MPC Growth MEM media. A higher concentration of calcium was observed in GENEA 02-MSC5 and BM-derived MSCs after 31 days of directed osteogenic differentiation when compared with the concentration of calcium detected in GENEA 02-MSC5 and BM-hMSCs (negative controls) cultured in MPC Growth MEM media during the same period of time.

(a)

(b)

(c)

(d)