TGF-β Inhibitor SB431542 Promotes the Differentiation of Induced Pluripotent Stem Cells and Embryonic Stem Cells into Mesenchymal-Like Cells

Figure 7

Morphological characteristics of GENEA 02-MSC5 (a), NF1-MSC3 (b), and BM-hMSCs (c) after 28 days of directed adipogenic differentiation. No signs of adipogenic differentiation were observed in GENEA 02-MSC5 and NF1-MSC3 cells after 28 days of directed adipogenic differentiation. The presence of lipid-rich vacuoles within cells was only observed in BM-derived MSCs. (d) Average fatty acid concentration in BM-hMSCs and ESC-derived MSCs after 28 days of culture in adipogenic differentiation/maintenance media or MPC Growth MEM media. A higher fatty acid synthesis was only observed in BM-derived MSCs after 28 days of directed adipogenic differentiation when compared with the concentration of calcium detected in GENEA 02-MSC5 cells subjected to standard adipogenic differentiation and GENEA 02-MSC5 and BM-hMSCs (negative controls) cultured in MPC Growth MEM media during the same period of time.

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