Azithromycin Promotes the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells after Stimulation with TNF-α

Figure 4

AZM restored the osteogenic potential of human PDLSCs through the WNT and NF-κB signaling pathways. PDLSCs were cultured in osteogenic medium and treated with 100 ng/ml TNF-α and AZM (10 μg/ml, 20 μg/ml) for 7 days. The protein levels of p65, phosphorylated p65, β-catenin, IκB-α, and phosphorylated IκB-α were detected by Western blot analysis. indicates significant differences. Data are presented as means ± SD.