Fish Scale-Derived Scaffolds for Culturing Human Corneal Endothelial Cells

Figure 4

Triple staining using HEC to determine live/dead cells. (a) Control cornea to show the presence of dead (red), live (green), and dying cells (blue without green marked with white arrows). Cell nuclei are shown in blue cells. (b) HCEnCs from old donor corneas cultured on Lab-Tek showed high viability and confluency without any dead cells. (c) Relatively lesser amount of cells was observed when HCEnCs were cultured on FSS. There were no dead cells observed on FSS too, but again, most of the cells were cultured in quadrants 2 and 3. HCEnCs: human corneal endothelial cell; FSS: fish scale scaffold.

(a)

(b)

(c)