Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation

Figure 2

Effects of the conditioned medium from MSCs on motor function and body weight of neuropathic mice. Bar graphs representing (a) the body weight variation of mice from different experimental groups at the end of the experimental period (60 days) and (b) the run time on the rotarod 24 h after treatments. Sham group represents mice without neuropathy, in which the sciatic nerve was exposed but left intact. Seven days after PSL mice were treated by endovenous route with bone marrow-derived mesenchymal cells (MSCs; 1 × 10⁶/100 μl), conditioned medium from MSCs (CM; 100 μl) or vehicle (control medium; 100 μl). Gabapentin (70 mg/kg), the reference drug, was administered twice daily by oral route for six consecutive days. Mice treated with diazepam (10 mg/kg), the reference drug of the rotarod test, were tested 1 h after treatment. Data are reported as means ± SEM; mice per group. Significantly different from the sham group (); significantly different from the vehicle-treated group (); significantly different from the remaining groups (). ANOVA followed by Tukey’s multiple comparison test.

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