Comparison of Immunological Characteristics of Mesenchymal Stem Cells from the Periodontal Ligament, Umbilical Cord, and Adipose Tissue

Figure 3

(a) Dose-dependent inhibition of activated PBMC proliferation by MSCs. PBMCs isolated from healthy donors were labeled with CFSE, stimulated with anti-CD3/anti-CD28 antibody-coated beads, and cocultured with naive MSCs at MSC : PBMC ratios of 1 : 25, 1 : 100, or 1 : 400 for 3.5 days. Proliferating cells were analyzed by flow cytometry, and the percentage of each experimental group is represented as a bar on the histogram. (b) Recovery of PBMC proliferation by inhibitors of IDO or COX-2. Identical experiments were performed as those in (a) at an MSC : PBMC ratio of 1 : 10 in the presence of the IDO inhibitor 1-methyl-L-tryptophan (1-MT, 0.1 mM), the COX-2 inhibitor (NS-398, 0.1 mM), or both. Flow cytometric profiles are shown in Supplementary Figure 2. All experiments were performed independently at least three times. The graph shows the means and standard errors of the mean (SEMs). values were obtained by ANOVA followed by Tukey’s post hoc test. and .

(a)

(b)