Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical Possibility

Figure 1

Expansion of γδ T cells. The number of viable cells after 14 days of culture is displayed according to culture conditions in (a). Here, and in (c) and (d), the grey box in the left graph encompasses the results of UCB γδ T cells cultured with 5 μM zoledronate and 50, 100, or 200 IU IL-2/ml medium, and in the in right graph, indicated by the arrow, the data inside the box is displayed separately for each cultured UB unit (each unit is indicated by a differently colored line). In (b) and (c), the proportion of γδ T cells is visualized in two different manners. In (b), the percentage of TCRγδ⁺ T cells is shown before and after culture. In (c), the same data is displayed for expansions of UCB cells using the bh-SNE algorithm to analyze and visualize the data. The panels to the left display the fluorescence intensity in the flow cytometry channel for TCRγδ, normalized using arcsin transformation with cofactor 150, for every cell in the displayed groups. In the top right panel, an overview of the cells in all samples is displayed and gated according to where the TCRγδ⁺ cells are clustered. The lower right panel shows the percentage of cells in each group found in the TCRγδ⁺ cell cluster. The fold expansion of γδ T cells is displayed in (d). IL-2: interleukin 2; TCR: T cell receptor; ZOL: zoledronate.

(a)

(b)

(c)

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