Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical Possibility

Figure 6

Marker expression in total T cells. Cosignaling marker expression. The phenotype of the cultured T cells from UCB and PB with regard to the expression of markers of activation and costimulatory and inhibitory receptors was assessed by flow cytometry. The percentage of cells expressing the costimulatory marker CD28 (a), the activation markers CD95 and CD69 (b), and the coinhibitory markers CTLA-4, PD-1, LAG-3, and TIM-3 (c) are displayed. In (d), the percentage of TCRαβ⁺ T cells before and after expansion is displayed in the left panel. The percentages of CD56⁺ CD3⁻ and CD56⁺ CD3⁺/viable cells are displayed in the middle panel of (d), and the right panel shows the percentage of CD19⁺ lymphocytes before and after culture. IL-2: interleukin 2; TCR: T cell receptor; ZOL: zoledronate.

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