Table 1: Primer sets for reverse transcription polymerase chain reaction.

NameSequenceAnnealing temp (°C)Size (bp)

GAPDHF: GCC AAA AGG GTC ATC ATC TC61379
R: ACG GAT ACA TTG GGG GTA GG
Oct4F: CCC GGA AGA GAA AGC GAA CT58362
R: GAC GGG AAC AGA GGG AAA GG
NanogF: AGG GTC TGC TAC TGA GAT GCT CTG56363
R: CAA CCA CTG GTT TTT CTG CCA CCG
LIFF: ATT GTG CCC TTA CTG CTG CT61583
R: GCC TGG ACC ACC ACA CTT AT
CT-1F: GAG GAA TAC GTG CAG CAA CA57389
R: AGC ACC TTG GCT GAG AAG AT
OSMF: CAC GGC TTC TAA GAA CAC TGC59547
R: CGA TGG TAT CCC CAG AGA AA
CNTFF: CTT TCG CAG AGC AAT CAC CT61579
R: CCC CAT AAT GGC TCT CAT GT
BMP2F: TCC ATC ACG AAG AAG CCG TG58465
R: CCA AAA GTC ACT AGC AAT GGC
BMP4F: AGG GCC AGC ACG TCA GAA TC57430
R: ACC TTG TCA TAC TCA TCC AGG
CNTFRαF: CTG TTT CCA CCG TGA CTC CT59802
R: TGG GAC ACT GGT CAA GAA GA
NodalF: GCC AGA CAG AAG CCA ACT GTG61324
R: TCA GAG GCA CCC ACA CTC CTC
GluR6F: CTG CAG CAC AGA GAG GAA CCA60488
R: ATA ACT TCC TCC ATG TGC CTC AC
BrachyuryF: GCT GAG ACT TGT AAC AAC CG55266
R: GCA AAG GAC TCT GAT TAA CTG C
TTRF: CCG TTC CAT GAA TTC GCG GAT60240
R: TTC ACG GCA TCT TCC TGA GC
AFPF: TTG CCT CCA CGT GCT GCC AGC61341
R: CGC CAG CTG CTC CTC TGT CAG