The Human Skin-Derived Precursors for Regenerative Medicine: Current State, Challenges, and Perspectives

Table 3

The protocols for neurogenesis of hSKPs.

Differentiation	Basic medium	Reagent (concentration)	Indication	Ref.
Neuron differentiation	Neurobasal or DMEM/F12 (3 : 1) medium	Brain-derived neutrophic factor (50 ng/ml), nerve growth factor (50 ng/ml), neurotrophiin-3 (10 ng/ml), FBS (1%–5%)	Differentiation for 2–4 weeks with 50% of the medium changed every 3-4 days	[6]
Schwann cell differentiation	Neurobasal or DMEM/F12 (3 : 1) medium	N2 supplement (1% or 2%), forskolin (4 μm or 5 μm), heregulinβ (10 ng/ml or 50 ng/ml), FBS (1%)	Differentiation for 2–4 weeks with 50% of the medium changed every 3-4 days	[6, 34]
Astrocyte differentiation	B27 neurobasal medium	FBS (3%–10%)	Differentiation for 3 weeks with 50% of the medium changed every 3-4 days	[32]

DMEM: Dulbecco’s modified Eagle medium; FBS: fetal bovine serum.