Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure

Table 1

Comparison of human studies using enzymatic breakdown with collagenase for ADMSC isolation from subcutaneous tissue. Phosphate buffer solution (PBS), Hank’s balanced salt solution (HBSS), bovine serum albumin (BSA), and Dulbecco’s modified eagle’s medium (DMEM).

Author	Collagenase	Concentration	Dilution media	Enzymatic duration
Cheng et al. [42]	Type 1	0.1%	PBS	60 minutes
Choudhery et al. [43]	Type 4	0.2%	PBS	20 minutes
Satish et al. [44]	Type 2	0.1%	HBSS/BSA	40 minutes
Kinoshita et al. [45]	Type 1	0.075%	PBS	30 minutes
Al-Saqi et al. [46]	Type 2	0.1%	Unspecified	45 minutes
Koellensperger et al. [47]	Type 1	0.15%	BSA	45 minutes
Najar et al. [48]	Type 1	0.1%	BSA	45 minutes
Cervelli et al. [49]	Type 1	0.1%	Unspecified	60 minutes
Wu et al. [50]	Type 1	0.1%	DMEM	90 minutes
Yang et al. [51]	Type 1	0.1%	PBS	60 minutes
Yu et al. [52]	Type 1	0.1%	DMEM	60 minutes
Tan et al. [53]	Type 2	1.0%	HBSS/BSA	50 minutes
Kilroy et al. [54]	Type 1	0.1%	PBS/BSA	60 minutes
Jeon et al. [55]	Type 1	0.1%	HBSS/BSA	60 minutes
Rodriguez et al. [39]	Unspecified	0.2%	DMEM/BSA	10 minutes
Devireddy et al. [56]	Type 1	0.1%	PBS/BSA	60 minutes