Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Lineage</td><td class="align_center">Histological staining</td><td class="align_center">qPCR gene expression</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">Osteoblasts</td><td class="align_center">Alizarin Red, Von Kossa</td><td class="align_center">COL 1A1, OCN, runx 2, ALP</td></tr><tr><td class="align_left">Adipocytes</td><td class="align_center">Oil Red O</td><td class="align_center">PPARG 1 and 2, C/EBP a and d</td></tr><tr><td class="align_left">Chondroblasts</td><td class="align_center">Alcian blue</td><td class="align_center">COL 2A1, SOX-9, ACAN</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Mesenchymal stem cell differentiation testing. COL: collagen; OCN: osteocalcin; ALP: alkaline phosphatase; PPARG: peroxisome proliferator-activated receptor gamma; C/EBP: CCAAT/enhancer-binding protein; ACAN: aggrecan.</div>

Stem Cells International

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Table 2: Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure 