The Effects of Conditioned Medium Derived from Mesenchymal Stem Cells Cocultured with Hepatocytes on Damaged Hepatocytes and Acute Liver Failure in Rats

Figure 3

The effects of different CM treatments on damaged hepatocytes in vitro. (a) The viability of normal and damaged L02 cells cultured in each CM was examined by MTT assay (, , and ). (b) LDH level in L02 cells cultured in each types of CM. (c) AST level in L02 cells cultured in each types of CM (, , and ). (d) Total protein secreted by L02 cells cultured in each types of CM (, , and ). (e) Comparison of the number of apoptotic L02 cells cultured with different types of CM (, , and ). The apoptosis of L02 cells cultured in different types of CM was determined by FACS analysis using annexin V-propidium iodide staining.

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