Intravenous Transplantation of Mesenchymal Stem Cells Reduces the Number of Infiltrated Ly6C⁺ Cells but Enhances the Proportions Positive for BDNF, TNF-1α, and IL-1β in the Infarct Cortices of dMCAO Rats

Figure 1

Ly6C⁺ cells in the ischemic core cortex. The number of Ly6C⁺ cells gradually increases at the IBZ of infarct dMCAO in rats. (a) Ly6C staining of the sham group. (b) Ly6C staining of the infarct area 3 h after the onset of ischemia. (c) 24 h. (d) 48 h. (e) Ly6C staining in the infarct area at 48 h. (f) CD45 staining in the same view field as (e). (g) DAPI nucleus staining. (h) Merged image of (e), (f), and (g), showing that Ly6C-positive cells are all CD45-positive. Arrow: double-labeled cells. (i) Cerebral infarct of the “ischemia + vehicle” group, as stained by triphenyltetrazolium chloride (TTC). Rectangle: the area where cell counting was performed. Dotted lines: the boundary of the infarct areas. Scale bar, 50 μm.

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