Chemical Activation of the Hypoxia-Inducible Factor Reversibly Reduces Tendon Stem Cell Proliferation, Inhibits Their Differentiation, and Maintains Cell Undifferentiation

Figure 2

HIF-1α chemical activation with DMOG. HIF-1α expression was analyzed by Western blot (a). The activation of HIF-1α was also analyzed measuring the expression of VEGF by real-time PCR (b). Western blots were quantified by densitometry and results expressed with arbitrary units. Real-time PCR data are expressed as fold change as compared to control cells without DMOG treatment. values were calculated using Student’s t-test. Only values < 0.05 are indicated: , , and .

(a)

(b)