Research Article

User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9

Figure 4

Validation of Eed knockout mESCs. (a) RT-qPCR results indicate that transcription was totally abrogated in Eed knockout clones generated by 4-OHT treatment. Relative quantification of mRNA levels was performed by the delta Ct (dCt) method and normalized to endogenous GAPDH. are shown. . (b) Compared to controls, H3K27me3 expression (red, by H3K27) was dramatically decreased in Eed knockout mESCs generated by Cre-LoxP recombination upon 4-OHT treatment. Blue indicates DAPI staining.
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