Research Article
User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9
Table 1
Targeting efficiency of LNL and FNFL cassettes.
(a) Sequential targeting of LNL and FNFL cassettes |
| | | Number of clones | Number of heteroinsertion | Number of homoinsertion | Efficiency of heteroinsertion | Efficiency of homoinsertion |
| Eed | LNL insertion | 88 | 17 | 4 | 19.3% | 4.5% | FNFL insertion | 190 | 33 | 5 | 17.4% | 2.6% | SRCAP | LNL insertion | 94 | 65 | 3 | 69.1% | 3.2% | FNFL insertion | 96 | 70 | 2 | 72.9% | 2.1% |
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(b) Simultaneous targeting of LNL and FNFL cassettes |
| | | Clones with LNL heteroinsertion | Clones with LNL homoinsertion | Clones with FNFL heteroinsertion | Clones with FNFL homoinsertion | Clones with simultaneous LNL and FNFL homoinsertion |
| 10 kb promoter | Number of clones Total clone number :432 | 194 | 16 | 165 | 24 | 1 | Efficiency | 44.9% | 3.7% | 38.2% | 5.5% | 0.2% |
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