Zebularine Promotes Hepatic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells by Interfering with p38 MAPK Signaling

Figure 2

Zebularine promoted the mRNA expression of AFP and ALB. Noninduced BMMSCs and primary rabbit hepatocytes (PRHs) were used as negative or positive controls. (a) On day 7, noninduced BMMSCs expressed little AFP; increased AFP mRNA expression was observed in 0 and 10 μM groups. On day 14, zebularine exhibited significant effect on AFP expression, with the highest expression level in 10 and 20 μM groups. (b) Noninduced BMMSCs expressed little ALB. The mRNA levels of ALB started to increase at 14 days after pretreatment of zebularine at concentrations of 10 and 20 μM. Exposure to zebularine exhibited significant effect on ALB expression after 21 days, with the highest expression level in the 20 μM group. Asterisk indicates compared with the control group; dagger indicates compared with the 0 μM (HGF) group.

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