Alginate/Hydroxyapatite-Based Nanocomposite Scaffolds for Bone Tissue Engineering Improve Dental Pulp Biomineralization and Differentiation

Figure 6

Alkaline phosphatase (ALP) activity and collagen type I release in dental pulp stem cells (DPSCs) in the presence of alginate/hydroxyapatite scaffolds. (a) Bar graph showing the enzymatic activity of ALP (U/ml) normalized on the protein content (μg/sample) after 1, 3, 7, 14, 21, and 28 days of culture of DPSC growth onto scaffolds. 3 and 28 d scaffold versus 1 d scaffold; 3 d scaffold + DM versus 1 d scaffold + DM. (b) The bar graph represents collagen type I secretion from DPSCs cultured on scaffolds with or without DM after 1, 3, 7, 14, 21, and 28 days. Results are normalized on values obtained from DPSCs cultured on scaffolds for 1 day without DM after being proportioned on the protein content (μg/sample). 14 and 28 d scaffold + DM versus scaffold and scaffold + DM. Scaffold = DPSC-laden scaffolds; scaffold + DM = DPSC-laden scaffolds in the presence of differentiation medium.

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