Optimizing Osteogenic Differentiation of Ovine Adipose-Derived Stem Cells by Osteogenic Induction Medium and FGFb, BMP2, or NELL1 In Vitro

Figure 1

Part of the colonizing forming unit assay made by the crystal violet methanol after seeding SVF cells in T75 flasks. (A) Seeding of 1·10⁵ SVF cells from sample 1 for 7 days. (B) Seeding of 1·10⁴ SVF cells from sample 2 for 12 days. (C) Seeding of 1·10⁴ SVF cells from sample 3 for 12 days. (D) Seeding of 1·10³ SVF cells from sample 2 for 12 days. Low density of CFU is seen, and black dots form counting.