Bone Morphogenetic Protein 6 Inhibits the Immunomodulatory Property of BMMSCs via Id1 in Sjögren’s Syndrome

Figure 3

BMP6 impaired immunomodulatory properties of BMMSCs and downregulated PGE2 via Id1. (a, c, d) Real-time RT-PCR results demonstrated a higher level of Id1 and Id4 mRNA expression in BMMSCs derived from NOD mice compared with BALB/c, while Id2 and Id3 transcripts showed no significant difference between two groups (a) (). (b, e, f) BMP6-treated BALB/c BMMSCs showed an increase in Id1, rather than in Id2, Id3, or Id4 mRNA expression () Knockdown of Id1 increased BMP6-induced Cox2 (g) and PGE2 (h) downregulation, as evidenced by real-time RT-PCR and ELISA, respectively. (, , , ). (i) CFSE assays indicated that BMP6 inhibited downregulation of T cells proliferation mediated by BMMSCs, while Id1 knockdown attenuated this effect (, ). N.S.: no significant difference.

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