In Vitro Murine Hematopoiesis Supported by Signaling from a Splenic Stromal Cell Line

Figure 4

Signaling pathways which control hematopoiesis. Cocultures were established for 28 days by overlay of Lin⁻ bone marrow cells over 5G3 stroma. (a) Soluble inhibitors of β-catenin (XAV939) and Wnt activation (WntC59) were added into triplicate flasks. (b) Imatinib and Plerixafor were added into cocultures to assess the effect on cell production over time. In (a) and (b), data shows % myeloid (CD11b⁺) cells produced relatively to input cell number using flow cytometry. Data reflect the mean ± SE (). annotates inhibitor concentration where cell production is significantly different from controls which contained no inhibitor. Data shown are reflective of two similar experiments.

(a)

(b)