Characterization and Optimization of the Seeding Process of Adipose Stem Cells on the Polycaprolactone Scaffolds

Figure 18

The representative images of human adipose stem cells seeding into PCL and PCL + 5% TCP after 21 days of culture. (a) Osteogenic differentiation of ASCs in 2D culture (density: 0.3 × 10⁶). (b) Osteogenic differentiation (ARS) in PCL scaffold. (c) Osteogenic differentiation in PCL + 5% TCP. (d) Adipogenic differentiation (Oil Red O) of ASCs in 2D culture. (e) Adipogenic differentiation in PCL scaffold. (f) Adipogenic differentiation in PCL + 5% TCP scaffold. Seeding density of ASCs: 0.9 × 10⁶ per scaffold. Representative images are shown at 10x magnification. Scale bars represent 100 μm. Images were taken by using an Olympus CKX41 microscope.