Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues

Figure 1

(a) Adipose tissue mesenchymal cells, (b) fibroblasts, and (c) umbilical cord tissue mesenchymal cells, 200x. After multilineage differentiation, it is possible to observe (a i, b i, and c i) adipogenic differentiated cells characterized by Sudan black-stained lipid vacuole accumulation, 200x; (a ii, b ii, and c ii) osteogenic differentiated cells characterized by calcium deposition, which were stained with alizarin red, 100x; and (a iii, b iii, and c iii) chondrogenic differentiated cells characterized by chondrogenic pellet development, which were stained with Alcian blue, 200x. Negative control cells maintained the typical spindle-like shape and differed from the treated cells.