Adipose-Derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities

Figure 3

Immunofluorescence analysis of ADSC marker expression after 7 days of culture in standard medium (undifferentiated ADSCs) and in adipogenic differentiation medium (differentiated ADSCs). (a) Immunofluorescence assay for C/EBPδ, PPARγ, adiponectin (AD), leptin receptor (LR), C/EBPβ, and FAB4 (green labelling) which are markers of adipogenesis progression. Nuclei were stained with DAPI (blue labelling). Negative control isotype staining was performed using normal goat serum in place of the primary antibody. Scale bars: 50 μm. (b) Quantification of cell positivity to adipogenic markers. The number of positive cells, expressed as the percentage to the total cell number given by DAPI nuclear staining, was calculated as an average of 15 different fields for each marker (3 fields/isolate). Error bars represent the SEM for three experiments. The statistical significance was determined by Student’s -test; vs. undifferentiated ADSCs; vs. undifferentiated ADSCs; vs. undifferentiated ADSCs.

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