Adipose-Derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities

Figure 5

Effect of PPARγ activation and FFA supplementation on BC ADSC differentiation. (a) Adipogenesis was revealed by Oil Red O staining for lipid droplets. (a) shows representative images of BC ADSCs in control conditions and after adipogenic differentiation for 7 days with three different protocols (differentiated BC ADSCs, differentiated BC ADSCs with troglitazone, and differentiated BC ADSCs with lipid mixture). Data are representatives of 5 different isolates. Scale bars: 200 μm. (b) Magnifications of (a) images of BC ADSCs in control conditions and after adipogenic differentiation for 7 days with three different protocols (differentiated BC ADSCs, differentiated BC ADSCs with troglitazone, and differentiated BC ADSCs with lipid mixture). Minimal lipid droplet accumulation after troglitazone supplementation is highlighted by red arrows. Data are representatives of 5 different isolates. Scale bars: 100 μm. (c) Percentage of cells positive to Oil Red O staining and quantification of Oil Red O extracted from lipid droplets measured at 520 nm normalized over nucleus counts. Data are reported as the of 5 different isolates; the statistical significance was determined by one-way ANOVA. and vs. UNDIFF BC ADSCs.

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