Adipose-Derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities

Figure 6

The effect of PPARγ inhibition on PF and BC ADSC differentiation. (a) Adipogenesis was revealed by Oil Red O staining for lipid droplets. (a) shows representative images of PF ADSCs and BC ADSCs in control conditions, after adipogenic differentiation for 7 days and after adipogenic differentiation for 7 days with the PPARγ inhibitor T0070907. Data are representatives of 5 different isolates. Scale bars: 200 μm. (b) Percentage of cells positive to Oil Red O staining and quantification of Oil Red O extracted from lipid droplets measured at 520 nm normalized over nucleus counts. Data are reported as the of 5 different isolates; the statistical significance was determined by one-way ANOVA. and vs. UNDIFF PF ADSCs; vs. DIFF PF ADSCs; ^°°°°, ^°°, and ^° vs. UNDIFF BC ADSCs; ^§§§§ and ^§§§ vs. DIFF BC ADSCs.

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