Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin

Figure 5

The ALP staining of the cells subjected to mechanical stretch with or without EPO was more obvious than that of the control (a). The group subjected to mechanical stretch combined with 20 IU/ml EPO yielded the largest ALP activity relative to the control (b). BMSCs subjected to mechanical stretch with or without EPO show a change in the mRNA expression levels of ALP, OCN, COL, and Runx2 compared to the control group. The mRNA expression of ALP in the group of mechanical stretch combined with 20 IU/ml EPO showed the maximum value, with the expression significantly increased about 5.37-fold compared to that of the control. The mRNA expressions of OCN, COL, and Runx2 in the group of mechanical stretch combined with 20 IU/ml EPO increased about 5.85-fold, 5.41-fold, and 5.42-fold, respectively, compared to those of the control (c). and compared with the control group.

(a)

(b)

(c)