Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia

Figure 1

Morphology, phenotypic markers, and multilineage capacities of MenSCs-PE. (a) Fibroblast-like morphology of MenSCs isolated from healthy women (control) and women with a previous preeclampsia (PE). MenSCs from PE showed the same morphology as MenSCs isolated from control. (b) Phenotypic characterization of MSC surface markers in MenSCs isolated from women with a history of PE (red) and control (black) and their respective isotype control (gray); the cells were analyzed by flow cytometry. All MenSCs from both groups were positive for CD90, CD73, CD105 (endoglin), and CD44 and negative for CD14, CD34, HLA-DR, and CD45. (c) Osteogenic differentiation of MenSCs isolated from healthy control women and women who developed PE was evaluated by measuring mRNA expression of RUNX2 and osteocalcin by qPCR. (d) Adipogenic differentiation of MenSCs isolated from healthy control women and women who developed PE was evaluated by measuring FABP4 and PPAR-γ by qRT-PCR. Results are (standard error of the mean). Statistical analysis was performed using Student’s -tests.

(a)

(b)

(c)

(d)