Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
Reduced expression and secretion of VEGF and reduced expression of endoglin on MenSCs-PE. (a) Representative VEGF Western blot of MenSCs isolated from healthy women (control) and women who developed preeclampsia (PE) cultured under conditions that recapitulate the decidualization in vitro. (b) Semiquantification of VEGF by Western blot of MenSCs from PE showed a significant lower protein expression of VEGF than that of MenSCs isolated from control women. (c) VEGF secretion measured by ELISA analysis of MenSCs from control and PE; MenSCs from women with past PE had significantly reduced secretion of VEGF compared to MenSCs isolated from control women. (d) Representative endoglin Western blot of MenSCs from control and PE cultured under conditions that recapitulate the decidualization in vitro. (e) Semiquantification of endoglin protein expression demonstrated significantly lower protein expression of endoglin in MenSCs from women with past PE compared to MenSCs isolated from control women. (f) Analysis of endoglin secretion demonstrated no significant difference in endoglin secretion in MenSCs from control and PE. Results are (standard error of the mean). Statistical analysis was performed using Student’s t-tests. and significant.