Research Article
Increased Neuronal Differentiation Efficiency in High Cell Density-Derived Induced Pluripotent Stem Cells
Figure 1
Characterization of induced pluripotent stem cells (iPSCs) generated from adult human neuroprogenitor cells (aNPCs). Both iPSC-aNPCLow and iPSC-aNPCHigh show (a) round colonies with no spontaneous differentiation observed around the border of the colonies. Scale bar is 250 μm and 100 μm for 10x and 20x magnifications, respectively. (b) Representative images of clones stained for surface markers including AP, SSEA4, and Tra 1-60 and cytoplasmic and transcription factors including LIN28A, OCT4, SOX2, and NANOG which are all markers for pluripotency. Clones are positive for all markers tested. Scale bar represents 100 μm. (c) In vitro germ layer differentiation of iPSC clones from both low and high densities stained for TUJ1, GATA4, and α-SMA representing ecto-, endo-, and mesoderm markers, respectively. Representative images are shown here. Scare bars are 100 μm and 50 μm.
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