Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa

Figure 1

Schematic illustration of the constructed vector using the porcine OCT4 promoter. (a) EGFP reporter vectors carrying different elements of the pig Oct4 URS were constructed from a pEGFP-N1 vector. One RFP reporter vector carrying a 1.7 kb pig Oct4 URS was constructed from a pDsRed2 vector. (b) Four constructs containing the different elements of the OCT4 promoter in the pGL3-basic vector and the pGL3-basic vector alone as a control were transfected into cells as described in Materials and Methods. DE: distal enhancer; PE: proximal enhancer. The deleted regions are shown as dotted lines.

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