Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa

Figure 3

Alignment of porcine OCT4 transcriptional regulatory regions with those of five other mammalian orthologs and schematic representation of the porcine OCT4 transcriptional regulatory region. (a) Conserved region 4 (CR4) contains distal enhancer site 2A (DE2A) and the binding domain for Oct4 and Sox2 (Oct4/Sox2). Conserved region 3 (CR3). Conserved region 2 (CR2) contains proximal enhancer site 1B (PE1B). Conserved region 1 (CR1) contains the Sp1/Sp3 site, hormone responsive element (HRE), and three G/C-rich sites. The asterisks show the nucleotides that are identical among the six species. (b) The black line represents the 3.5 kb upstream region (located at positions -3500/-1) of OCT4 in pig. The four upper lines of CR1, CR2, CR3, and CR4 represent the four conserved regions, respectively. The circles show the locations of distal enhancer site elements 2A (DE2A) and 2B (DE2B) and the proximal enhancer site elements 1A (PE1A) and 1B (PE1B). The arrow indicates the translational initiation site of the OCT4 gene.

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