A High-Throughput Method as a Diagnostic Tool for HIV Detection in Patient-Specific Induced Pluripotent Stem Cells Generated by Different Reprogramming Methods

Figure 1

Generation and characteristics of hiPSCs derived from skin fibroblasts (FB). (a) Reprogramming efficiency for generation of PMBC-hiPSCs and FB-hiPSCs based on different reprogramming methods. Reprogramming efficiency was evaluated as total iPSC colonies obtained up to day 25 from 4.8 to starting FB (dependent on the used method); (p-FB = plasmids), (STv-FB = STEMCCA), and (sv-FB = Sendai virus). PBMCs were used for reprogramming by sv; (sv-PBMCs). ; . (numbers) are defined as reprogramming experiments of different somatic cell sources by the mentioned method. (b) Reprogramming efficiency for generation of FB-iPSCs (), PBMC-iPSCs (), and MSC-iPSCs using episomal plasmids. MSCs were used for reprogramming by plasmids; . (c) Morphology of iPSCs. iPSCs show human ES-like morphology and are positive for alkaline phosphatase (ALP). Scale bar: 100 μm.

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