Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3D

Figure 6

Flow cytometry analysis of α10 integrin and IIB procollagen expression. After expansion in monolayer under serum-free conditions, P1 BM-MSCs and nasal chondrocytes were seeded in agarose hydrogel. The cell-agarose constructs were then cultivated under serum-free conditions for 21 days in the presence of BTβ3 (for BM-MSCs) or BIT (for chondrocytes). The cells were released from the constructs by agarase digestion and analyzed by flow cytometry. Percentage of cells expressing (a) type IIB procollagen or (b) α10 integrin. Data are presented as box plots with median as a bar (). (c) Single-stained populations (α10^-/IIB⁺ and α10⁺/IIB^-) and double-stained populations (α10⁺/IIB⁺) are present in relatively equivalent proportions in BM-MSCs and chondrocytes, as reported in the quadrants (percentage of positive cells is represented as , ). Red contour: IIB procollagen; blue contour: α10 integrin.

(a)

(b)

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