Stem Cells International / 2019 / Article / Fig 1

Research Article

Schwann Cell-Like Cells Derived from Human Amniotic Mesenchymal Stem Cells Promote Peripheral Nerve Regeneration through a MicroRNA-214/c-Jun Pathway

Figure 1

c-Jun is a direct target of miR-214. (a) Schematic illustration of the hypothetical duplexes formed through interactions between the binding site in the c-Jun 3-UTR and miR-214. (b) RT-qPCR analysis of miR-214 levels in hAMSCs and SCLCs. (c) RT-qPCR analysis of miR-214 levels in SCLCs that were transfected with nc, miR-214, or anti-miR-214. (d, e) Western blotting analysis of c-Jun protein levels in SCLCs that were transfected with nc, miR-214, or anti-miR-214. GAPDH was used as the internal control: (d) representative image; (e) quantitative analysis. (f) Relative luciferase signals from SCLCs that were cotransfected with luciferase reporter gene (wt) or mutated luciferase reporter gene (mut) and nc, miR-214, or anti-miR-214. Data are shown as (). , relative to nc.
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