Schwann Cell-Like Cells Derived from Human Amniotic Mesenchymal Stem Cells Promote Peripheral Nerve Regeneration through a MicroRNA-214/c-Jun Pathway

Figure 5

c-Jun negatively regulates the migration and myelination of SCLCs. c-Jun was overexpressed or knocked down in SCLCs through transfection with c-Jun plasmid or c-Jun siRNA, respectively. (a, b) Western blotting analysis of c-Jun protein levels in SCLCs that were transfected with c-Jun plasmid or c-Jun siRNA. GAPDH was used as the internal control: (a) representative image; (b) quantitative analysis. (c) Migration rates of SCLCs that were transfected with c-Jun plasmid or c-Jun siRNA. (d, e) Western blotting analysis of Egr2, MBP, and MPZ in SCLCs that were transfected with c-Jun plasmid or c-Jun siRNA: (d) representative image; (e) quantitative analysis. Data are shown as (). , relative to control.

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