Local Application of Semaphorin 3A Combined with Adipose-Derived Stem Cell Sheet and Anorganic Bovine Bone Granules Enhances Bone Regeneration in Type 2 Diabetes Mellitus Rats

Figure 3

Characterization of ASCs. (a) Primary culture of ASCs (original magnification ×100). (b) Subculture of ASCs (P3, original magnification ×100). (c) Mineral node stained with Alizarin Red S (original magnification ×40). (d) Fat droplets stained with Oil Red O (original magnification ×200). (e) Flow cytometry analysis of ASC surface markers.

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