Pharmacological Transdifferentiation of Human Nasal Olfactory Stem Cells into Dopaminergic Neurons

Table 1

Antibodies used for immunostaining and western blot.

	Antibodies	Species	Concentration	Origin	Catalog number
Primary antibodies	βIII-tubulin	Rabbit	IF: 1/1000	Covance	PRB-435P
	Calbindin	Rabbit	IF: 1/200	Abcam	ab25085
	Dopamine	Rabbit	IF: 1/500	Abcam	ab6427
	Dopa decarboxylase	Rabbit	WB: 1/1000; IF: 1/200	Abcam	ab3905
	Dopamine D2 receptor	Goat	IF: 1/200	Abcam	ab30743
	Dopamine transporter	Rat	WB: 1/1000; IF: 1/200	Millipore	MAB369
	Engrailed 1	Rabbit	IF: 1/25	Abcam	ab70993
	Foxa2/HNF3b	Rabbit	WB: 1/1000; IF: 1/400	Cell Signaling	8186
	Girk2	Goat	IF: 1/100	Abcam	ab65096
	Lmx1a	Rabbit	WB: 1/1000; IF: 1/200	Millipore	AB10533
	MAP2	Chicken	WB: 1/10000; IF: 1/5000	Abcam	ab5392
	Nurr1	Mouse	IF: 1/300	Abcam	ab41917
	Pitx3	Rabbit	WB: 1/250; IF: 1/200	Abcam	ab30734
	TH	Rabbit	WB: 1/500	Millipore	AB152
	TH	Rabbit	IF: 1/500	Pel-Freez	P40101-150
	Actin-HRP	Mouse	WB: 1/10000	Proteintech	HRP-60008
Secondary antibodies	Rabbit-HRP	Goat	WB: 1/6000	Cell Signaling	7074
	Chicken-HRP	Goat	WB: 1/6000	Abcam	ab97135
	Rat-HRP	Goat	WB: 1/5000	Millipore	AP136P
	Anti-rabbit-Alexa 488	Goat	IF: 1/1000	Thermo Fisher	A11070
	Anti-goat-Alexa 488	Donkey	IF: 1/1000	Thermo Fisher	A11055
	Anti-mouse-Alexa 488	Goat	IF: 1/1000	Invitrogen	A11029
	Anti-rat-Alexa 488	Goat	IF: 1/1000	Thermo Fisher	A11006
	Anti-chicken-Alexa 647	Goat	IF: 1/1000	Thermo Fisher	A21449
	HCS CellMask Orange		IF: 1/20000	Thermo Fisher	H32713
	DAPI		IF: 1/10000	Sigma Aldrich	D8417

WB: western blot; IF: immunofluorescence; HCS: high-content screening.