Research Article
Pharmacological Transdifferentiation of Human Nasal Olfactory Stem Cells into Dopaminergic Neurons
Table 1
Antibodies used for immunostaining and western blot.
| | Antibodies | Species | Concentration | Origin | Catalog number |
| Primary antibodies | βIII-tubulin | Rabbit | IF: 1/1000 | Covance | PRB-435P | Calbindin | Rabbit | IF: 1/200 | Abcam | ab25085 | Dopamine | Rabbit | IF: 1/500 | Abcam | ab6427 | Dopa decarboxylase | Rabbit | WB: 1/1000 IF: 1/200 | Abcam | ab3905 | Dopamine D2 receptor | Goat | IF: 1/200 | Abcam | ab30743 | Dopamine transporter | Rat | WB: 1/1000 IF: 1/200 | Millipore | MAB369 | Engrailed 1 | Rabbit | IF: 1/25 | Abcam | ab70993 | Foxa2/HNF3b | Rabbit | WB: 1/1000 IF: 1/400 | Cell Signaling | 8186 | Girk2 | Goat | IF: 1/100 | Abcam | ab65096 | Lmx1a | Rabbit | WB: 1/1000 IF: 1/200 | Millipore | AB10533 | MAP2 | Chicken | WB: 1/10000 IF: 1/5000 | Abcam | ab5392 | Nurr1 | Mouse | IF: 1/300 | Abcam | ab41917 | Pitx3 | Rabbit | WB: 1/250 IF: 1/200 | Abcam | ab30734 | TH | Rabbit | WB: 1/500 | Millipore | AB152 | TH | Rabbit | IF: 1/500 | Pel-Freez | P40101-150 | Actin-HRP | Mouse | WB: 1/10000 | Proteintech | HRP-60008 |
| Secondary antibodies | Rabbit-HRP | Goat | WB: 1/6000 | Cell Signaling | 7074 | Chicken-HRP | Goat | WB: 1/6000 | Abcam | ab97135 | Rat-HRP | Goat | WB: 1/5000 | Millipore | AP136P | Anti-rabbit-Alexa 488 | Goat | IF: 1/1000 | Thermo Fisher | A11070 | Anti-goat-Alexa 488 | Donkey | IF: 1/1000 | Thermo Fisher | A11055 | Anti-mouse-Alexa 488 | Goat | IF: 1/1000 | Invitrogen | A11029 | Anti-rat-Alexa 488 | Goat | IF: 1/1000 | Thermo Fisher | A11006 | Anti-chicken-Alexa 647 | Goat | IF: 1/1000 | Thermo Fisher | A21449 | HCS CellMask Orange | | IF: 1/20000 | Thermo Fisher | H32713 | DAPI | | IF: 1/10000 | Sigma Aldrich | D8417 |
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WB: western blot; IF: immunofluorescence; HCS: high-content screening.
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