Figure 6: aNFC allows calcium imaging in 3D. (a) Fluorescence time lapse images were collected for 6 min, recording spontaneous cytosolic calcium oscillations in ADSCs within 0.2% aNFC. The fluorescence intensity of four individual ADSCs was quantified (coloured boxes). (b, c) Quantification of fluorescence intensity (b) shows unsynchronized cytosolic calcium oscillations (c). Bar in (a): 25 μm; bar in (c): 12.5 μm.