Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker

Figure 1

Establishment and verification of the immortalized cell line SHED-Bmi1-EGFP from primary SHED. (a) Representative image of colonies formed after 3 d of isolation. Scale bar, 200 μm. (b) Representative images of nontransfected original cells (SHED-ori) and SHED-Bmi1-EGFP with different passages. Scale bar, 200 μm. (c) Protein level of Bmi-1 in SHED-ori P4 and SHED-Bmi1-EGFP P40 was detected by Western blot. The relative protein expression levels were normalized to β-actin. (d) Expression levels of Bmi-1 were assayed with qRT-PCR. (e) Expression levels of Nanog and Oct4 were assayed with qRT-PCR. (f) Population doublings (PDLs) of SHED-ori and SHED-Bmi1-EGFP. Data are shown as the of 3 separate experiments. , , and

(a)

(b)

(c)

(d)

(e)

(f)