Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker

Figure 2

Senescence level and proliferation capacity of SHED-ori and SHED-Bmi1-EGFP. (a) Blue cells were counted as a measure of senescence. Scale bar, 100 μm. (b) SHED-ori were stained with β-gal at P4 and P20. SHED-Bmi1-EGFP were stained at P40. Blue cells were counted as a measure of senescence. Scale bar, 200 μm. (c) Colony formation assay was performed to measure the proliferation capacity of SHED-ori P4, SHED-ori P20, and SHED-Bmi1-GFP P40.

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