Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker

Figure 4

Assessment of potential tumourigenicity ability of SHED-Bmi1-EGFP. (a) Cytogenic analysis of immortalized cells. SHED-Bmi1-EGFP P40 showed no abnormality of karyotype. (b) Assessment of potential tumourigenicity of SHED-Bmi1-EGFP in vivo. In a total of 5 weeks after injections of cells into the left forelimb armpit, no tumour formation was seen in PBS, SHED-ori P4, or SHED-Bmi1-EGFP P40 group. (c) Tumour slides stained with haematoxylin and eosin dye. Positive control showed heteromorphism while PBS, SHED-ori P4, and SHED-Bmi1-EGFP P40 did not. Scale bar, 100 μm.

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